Fricke, Nicolai Felix (2009) Der Nachweis endokrin wirksamer Substanzen in der Ostsee anhand der Messung der Vitellogenin-Konzentration im Blutplasma männlicher Dorsche (Gadus morhua). (Diploma thesis), Christian-Albrechts-Universität zu Kiel, Kiel, Germany, 100 pp.

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Abstract

The Baltic Sea is considered to be one of the most polluted oceans in the world. The low water exchange with the North Sea and the large catchment area, in addition with a correspondingly high anthropogenic pollution, are the key causes for the high pollution of this region. Implications of these pollutants were, next to processes of eutrophication, noxious effects for the Baltic Sea organisms. A comparatively neglected field of the analysis of pollutants in the Baltic Sea are the implications of hormone-active pollutants (EDC). Due to its mode of action this type of pollutant is able, even in minimal doses, to result in development disruptions or procreation damages for fish species. An eminently important focal point of the analysis of EDC has been the feminization of male fish species. Here, the vitellogenin (VTG), a crucial precursor of the yolk protein, appeared as a significant biomarker in the measurement of EDC effects in an aquatic environment. On this account this project developed a competitive ELISA on the ground of existing projects which rendered the classification of VTG concentrations in the serum of male cod (Gadus morhua) possible. Moreover, a part of the DNA was sequenced to gather more information about the functioning of this protein...

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