Antibiotic treatment of the breadcrumb sponge Halichondria panicea and subsequent recolonization V.2.

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Schmittmann, Lara and Hentschel, Ute (2022) Antibiotic treatment of the breadcrumb sponge Halichondria panicea and subsequent recolonization V.2. Open Access . protocols.io, 15 pp. DOI 10.17504/protocols.io.3byl4kpk2vo5/v2.

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Abstract

This protocol generates sponges (Halichondria panicea) with a disturbed microbiome under controlled experimental conditions, in order to study bacterial recolonization dynamics. Bacteria-bacteria interactions can be analysed with this set-up within the host environment aiming at a better understanding of sponge-microbe symbiosis in vivo.

It is divided into the sections 1) preparation, 2) antibiotic treatment and recovery phase, 3) recolonization with the natural microbiome and 4) sampling.

Document Type: Report (Protocol)
Keywords: host-microbe; interactions; sponge model; symbioses; microbiome; metaorganism; holobiont; antibiotic treatment
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MS Marine Symbioses
Main POF Topic: PT6: Marine Life
Publisher: protocols.io
Date Deposited: 09 Feb 2023 10:16
Last Modified: 09 Feb 2023 10:16
URI: https://oceanrep.geomar.de/id/eprint/57960

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