Freese, Marko (2010) The application of biotechnologically produced nematodes as live feed organisms for early life stages of shrimps and prawns. (Diploma thesis), Christian-Albrechts-Universität zu Kiel, Kiel, Germany, 78 pp.

Text Dipl. 2010 Freese, M.pdf - Reprinted Version Restricted to Registered users only Available under License German copyright act UrhG. Download (7MB) | Contact

Abstract

Aquaculture is the fastest growing sector of the entire food industry. lncreasing demands for seafood in the last decades led to an enormous increase in production of aquatic organisms. Especially the cultivation of prawns and shrimps reached unprecedented yields. Most of the economically important shrimps and prawns are dependent on live feeds, which neither are available in sufficient amounts at all times nor provide consistent quality. For this reason the finding of live feed alternatives had drawn much scientific attention in the recent past. Feeding experiments with larval shrimp and rhabditid nematodes showed promising results. Nevertheless nematodes as live feeds have yet not been adopted by the aquaculture industry. The reasons for this lay in deficiencies of certain nutrients in the nematodes and the abstinence of convenient mass culturing techniques. In the present study, different enrichment techniques of liquid media for the enhancement of fatty acid composition in the nematode Panagrellus redivivus were compared and assessed. Furthermore, the microorganisms Escherichia coli and Saccharomyces cerevisiae were tested for their suitability in nematode production. The present study revealed that the production yields of the free living nematode Panagrellus redivivus were significantly higher when the yeast Saccharomyces cerevisiae was applied, compared to yields achieved with Escherichia coli. Results evidenced that the formulation of liquid media had direct influence on the fatty acid composition as well as the production output of cultivated nematodes. Also, fractions of long-chained, polyunsaturated fatty acids in nematodes were significantly altered by the application of fish oil into the liquid media as well as by the addition of microalgae. Additionally, evidence was provided that larvae of the freshwater prawn Macrobrachium lanchesteri are capable of capturing and ingesting nematodes. The similarities in their larval development to the important aquaculture species Macrobrachium rosenbergii as well as the here discussed attributes qualify this species as an appropriate model organism for dietary studies with prawn larvae.

Actions (login required)

View Item